135Hz极低频电磁场对Hep G

作者：黄小军，杨家骥，王春梅，黄晓峰，李珍，曹云新，于华，王爽，陈丹
【关键词】 细胞凋亡
Growth and apoptosis of Hep G2 cells affected by extremely lowfrequency electromagnetic field
　　【Abstract】AIM: To investigate the effects of exposure to extremely lowfrequency electromagnetic field (ELF) on the growth and apoptosis of Hep G2 cells. METHODS: Hep G2 cells were exposed to a 135Hz ELF for 6, 12, 24 h respectively and then the cellular viability, cell cycle and percentage of apoptotic cells in the treated Hep G2 cells were measured respectively by methe thiazolyl tetrazolium (MTT) reduction assay and flow cytometry. The ultrastructural changes were observed by scanning and transmission electron microscopy. RESULTS: The absorbent value（A） was greatly decreased in Hep G2 cells exposed to ELF, compared with that in the corresponding control groups (P